Target
Sample
Mean Cq
Mean Efficiency Corrected Cq
Normalized Expression
Relative Normalized Expression
Regulation
Compared to Regulation Threshold
bcl2
5 Fu
29.36
29.36
1.61248
0.07412
13.49113-
Down regulated
bcl2
(-ve) Control
28.86
28.86
21.75417
1
1
No change
bcl2
AgNPs
32.44
32.44
0.46529
0.02139
46.75447-
Down regulated
caspase 3
5 Fu
N/A
N/A
N/A
N/A
N/A
No change
caspase 3
(-ve) Control
N/A
N/A
N/A
N/A
N/A
No change
caspase 3
AgNPs
N/A
N/A
N/A
N/A
N/A
No change
GAPDH
5 Fu
30.05
30.05
N/A
N/A
N/A
No change
GAPDH
Control
33.3
33.3
N/A
N/A
N/A
No change
GAPDH
AgNPs
31.34
31.34
N/A
N/A
N/A
No change
p53
5 Fu
23.51
23.51
93.18246
2.04442
2.04442
No change
p53
(-ve) Control
27.79
27.79
45.57886
1
1
No change
p53
AgNPs
27.36
27.36
15.80025
0.34666
2.88469-
No change
Survivin
5 Fu
27.53
27.53
5.75725
0.03707
26.97446-
Down regulated
Survivin
(-ve) Control
26.02
26.02
155.2988
1
1
No change
Survivin
AgNPs
34.17
34.17
0.14034
0.0009
1106.62206-
Down regulated
Table 3:
Gene expression regulation via the biogenic AgNPs treatment.
