Figure 4: Purification of anti-HER2 scFv. 12.5 % SDS-PAGE confiming the purification of anti-HER2scFv (31 kDa), CoomassieBrillian Blue R-25 stained gel; standard protein marker (M), total protein of culture extract lane 1 and scFv protein lanes 2, 3, and 4 are shown.