System design Detection strategy LOD

Target

 Ref.
Microfluidic single chip consists of seven PDMS microchambers with on-chip RNA purification, RT-LAMP and optical detection RT-LAMP and optical detection of turbidity change 35 pg RNA extracted from CymMV-infected Phalaenopsis orchids [59]
LAMP combined with lateral-flow dipstick Lateral flow dipstick < 5 pg IS6110 gene of M. tuberculosis 178 bp fragment [60]
PDMS based microfluidic channels and membrane for three microchambers with on-chip RNA extraction and RT-LAMP Real-time fluorescence 10-100 fg cDNA of NNV RNA1 [53]
“µ-LAMP”; PDMS-glass hybrid microfluidic chip with eight 5 µl microchannels and on-chip real-time absorbance detection device by integrated optical fibers Naked eye and real-time optical detection of turbidity change 10 fg PRV genomic DNA ~ 108 bp fragment (Hinc II restriction enzyme digestion assay) [50]
Magnetic bead-based microfluidic chip; five PDMS microchambers with on-chip cell lysis and DNA hybridization Spectrophotometric analysis (OD260) 10 fg DNA of MRSA [54]
Ten microchambermμLAMP system in a PDMS-glass format Direct naked-eye determination and SYBR green I fluorescence < 10 copies Conserved DNA fragments of three human influenza A substrains and eight important swine viruses [50]
CCD-based fluorescence imaging system in disposable COP-microchips; for ds-DNA standard dilution series: 16 circular wells with 1 mm diameter and 2 μL volume per well; for real-time LAMP: Seven V-shaped reaction wells with a volume of 2 μL per well; fabricated with 100 μm thick ZeonorFilm® Real-time detection; fluorescence imagining Single copy Genomic DNA from 12 virulence genes of major waterborne pathogens [61]
Novel SPR-LAMP microfluidic cartridge integrated with a polycarbonate-based prism coated with a 50 nm Au film Surface plasmon resonance (SPR) 2 fg HBV fragment [58]
Oxidized silicon well array (4 arrays of 6x6 wells) with dehydrated primers were covered with mineral oil; droplets (30 nL) are arrayed with an automated microinjection system Real-time fluorescence of EvaGreen® Not specified stx2 for E. coliO157, hlyA for Listeria monocytogenes, and invA for Salmonella [62]
6x8 semisolid polyacrylamide gel post array; each post: 670 nL volume; Peltier element for heating, a diode laser as an excitation source, and a CCD camera for detecting fluorescence in real-time Real-time fluorescence of the dye LCGreen Plus+, polymerized into the gel 63 fg Six M. tuberculosis DNA samples with variable concentrations and target numbers of IS6110 [63]
46 mm x 36 mm x 3.4 mm cassette consists of three solvent-bonded layers of PC; valve was formed with a composite of PDMS and highly expandable microspheres; on-chip real-time monitoring Real-time fluorescence with a portable, compact detector 10 copies E. coli DNA/RNA-fragments [64]
PMMA cartridge; exothermic reaction between Mg–Fe alloy and water as the heat source; reaction rate is controlled by using a filter paper; the amplification chambers’ temperatures are regulated with a phase change material Visual fluorescent detection with SYTO® 9 Green by naked eye and/or recorded with a portable digital camera 10 copies Fragment of E. coli [52]
FTA (Flinders Technology Associates (Whatman FTA®) membrane) disc was installed in the amplification reactor and operated in a flow-through (filtration) mode; DNA (captured on the FTA disc) were directly used as templates for LAMP without a need for elution and transfer of nucleic acids Blue LED excitation light for end-point detection with a cell phone camera Not specified Genomic DNA from mosquito tissue (Anopheles gambiae and An. grabiensis) [65]
Single reaction chamber with an integrated, flow-through FTO membrane for isolation, concentration and purification of DNA/RNA; thermal control by an external film heater Portable optical detection system < 10 HIV particles HIV particles suspended in raw saliva [66]
Table 1: Comparison between different LAMP devices.