| System design |
Detection strategy |
LOD |
Target |
Ref |
| Thermoformed foil-based centrifugal microfluidic lab-on-chip cartridge including prestored liquid and dry reagents, and a commercial available analyzer for incubation at 37 °C; separate 10 µL microchambers for amplifying up to 30 samples in parallel |
Real-time fluorescence |
< 10 copies |
mecA of Staphylococcus aureus |
[68] |
| Phase-quided microfluidic mixing chip; passive laminar flow mixing of two 6.5 µL batches in a microfluidic chamber |
Inverted fluorescence microscope |
Not specified |
Not specified |
[69] |
| Paper and plastic device assembled by stacking components made of acetate, double-sided adhesive, glass fiber matrix, and cellulose |
Lateral flow strips; visual detection |
10 copies |
HIV gag DNA |
[70] |
| Microfluidic digital RPA SlipChip; 1550 reaction compartments of 9 nL each, with 2 sets of wells for controls (50 wells each) |
Yes or no digital readout of end-point fluorescence |
300 copies/mL |
mecA of Staphylococcus aureus |
[71] |
