Samples OA loading(OA/protein molar ratio)2 Diameter (nm)3,5 Polydispersity index4 z-Potential (mV)5 Cyt c-OA1 12 ± 2 122.9 ± 5.5 0.174 ± 0.013 -0.489 ± 3.2 Cyt c       18.4 ± 4.0 BSA-OA1 53 ± 5 169.0 ± 4.6 0.416 ± 0.019 -81.3 ± 13.8 BSA       -27.4 ± 4.9

1Syntheses were performed in Tris-HCl buffer at pH 8 and 45°C. 2Number of OA attached on average to each protein molecule determined by FA quantification assay. 3This size was considered the upper limit for particle size determined by DLS. 4Polydispersity is used as a measure of the homogeneity of the NP. 5For these analyses, native Cyt c and FA-depleted B SA were dissolved and HAMLETlike complexes were suspended in filtered nanopure water

Table 1: Characterization of HAMLET-like complexes.