Figure 6: DAPI/PI staining and cellular uptake in A549 cells after 24 h of incubation. A) Co-localization of DAPI (blue) and PI (red) indicate cell death. Controls (Cyt c, first row, and HA, second row) do not cause cell death while Cyt c-HA 1MDa caused significant cell death. B) Cellular uptake of Cyt c (b), HA (c), and Cyt c-HA 1MDa (d). Autoflourescence of A549 cells is observed in a. Bar scale=20 μm.