Figure 2: Pharmacokinetics of 3H-nanozyme loaded to BMM or cell-free in the blood in MPTP-intoxicated mice. Mice were injected into tail vein with BMM loaded with nanozyme (black diamonds) or cell-free nanozyme (white squares). Following various time intervals after injection, mice were sacrificed and the whole blood samples (100μl) were taken into heparincoated microhematocrit tubes, and immediately centrifuged to obtain plasma. The levels of radioactivity were recorded, and the nanozyme concentration was expressed in μg catalase/mL plasma. Each point represents the mean ± S.E.M. of five mice per time point. The nanozyme organ content was expressed in μg catalase/mg tissue. Five mice were used for each treatment group. BMM loaded ex vivo with nanozyme, and administered into the blood stream play a depot role for the drug, providing considerably greater time of circulation compared to the cell-free catalase nanoformulation.