Table 1

Sample	Nominal Size (nm)	DLS, Z-Avg (nm)	TEM (nm)
20 nm silver nanoparticles
untreated	20	34.0 ± 0.4	36.4 ± 5.1
autoclaved	-	33.4 ± 0.2	37.2 ± 5.7
g- irradiated 15kGy g- irradiated 25kGy g- irradiated 50kGy	-	Polydispersed Polydispersed polydispersed	irregular shape irregular shape irregular shape
40 nm silver nanoparticles
untreated	40	38.3 ± 0.5	55.9 ± 9.6
autoclaved	-	37.8 ± 0.2	54.9 ± 8.4
g- irradiated 15kGy g- irradiated 25kGy g- irradiated 50kGy	-	Polydispersed Polydispersed polydispersed	irregular shape irregular shape irregular shape
60 nm silver nanoparticles
untreated	60	65.3 ± 0.4	78.3 ± 12.3
autoclaved	-	67.5 ± 1.0	74.6 ± 10.5
g- irradiated 15kGy g- irradiated 25kGy g- irradiated 50kGy	-	Polydispersed Polydispersed polydispersed	irregular shape irregular shape irregular shape
80 nm silver nanoparticles
untreated	80	90.7 ± 0.3	107.2 ± 14.1
autoclaved	-	90.0 ± 0.6	109.3 ± 13.2
g- irradiated 15kGy g- irradiated 25kGy g- irradiated 50kGy	-	Polydispersed Polydispersed polydispersed	irregular shape irregular shape irregular shape

Table 1: Summary of size analysis of silver nanoparticles before and after sterilization treatments by autoclave and various doses of gamma irradiation. Note: numbers after the ± symbol represent standard deviations. For the DLS measurements, these values indicate the reproducibility of the measurement (n > 12), not the polydispersity of the size distribution