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Figure 3: Effects of irradiation on the microbial composition. A: DGGE profiling of the oral microbiota present in the apical compartment after 24 h of co-culture with TR146 epithelial cells in irradiated (10 Gy) or non-irradiated (0 Gy) conditions. Indicated (arrow) is a group of GC-poor bacteria present in all samples (n=24) B: Pyrosequencing analysis of the oral microbiota cultured for 24 or 48 h in co-culture with TR146 epithelial cells in irradiated or non-irradiated conditions. Results of 454 pyrosequencing of 16S rDNA assigned to genus are shown. |