Figure 2: Diagram of the SCN1A reporter and neuron selection strategy for electrophysiological analyses: In the construct diagram, the yellow portion indicates the 5’-untranslated exonic sequence, and light blue indicates the 5’-untranslated sequence of the first coding exon. This construct was transduced into the dissociated neurospheres by using lentiviral vectors. After 3–7 weeks of terminal differentiation, putatively mature neurons that satisfied several requirements (see main text) were selected for electrophysiological analysis. Functionally immature neurons with in adequate action potential generation were excluded from analysis. APs, action potentials; RMP, resting membrane potential.