Figure 5: CNB-001 tPA interaction dose-response curves (IC50 extrapolation): Effect of CNB-001 (7 nM to 7 μM) on tPA activity measured in vitro compared to control. The results presented in the form of both 5 (dashed line- closed square) and 10 minutes (solid line-closed triangle) tPA assays. The diagonal lines are linear regression curve fits in order to extrapolate IC50 values. Control values for the assays are OD=0.62 and 0.90 for the 5 and 10 minute assays, respectively. Extrapolated IC50 values are 65.2 and 704 μM, respectively for the 5 and 10 minute assays.