Figure 3: Guanosine 5’-[βthiol]diphophate (GDPβS) induced potentiation of the excitatory postsynaptic current (EPSC) amplitude is blocked by protein kinase A (PKA) inhibitor Rp-3’,5’-cyclic monophosphothioate triethyl- amine (Rp-cAMP). A: Graph of EPSC amplitude during the first 10 minutes of recording showing no significant change in the amplitude with time. Insets are representative traces of EPSCs in the beginning (1) and after 10 minutes (2) of recording. B: Graph of the amplitude of EPSC recorded with a solution containing GDPβS versus time, revealing a significant increase in EPSC amplitude in the presence of GDPβS. Insets in the top are representative traces of EPSCs in the beginning (1) and after 10 minutes (2) of recording. C: Rp-cAMP antagonises the effect of GDPβS, and the EPSC amplitude change with time that occurs without Rp-cAMP does not occur in the presence of the GDPβS. Insets in the top are representative traces of EPSCs in the beginning (1) and after 10 minutes (2) of recordings. Error bar represent the sem.