Figure 2: Immunostaining with IgM anti-GalNAc-GD1a mAb incross-sectional and longitudinal sections of rat hemi-diaphragms.(A) Staining with IgM anti-GalNAc-GD1a mAb (green) overlappedwith the postsynaptic acetylcholine receptor cluster marker, α-bungarotoxin (α-BuTx, red), and anti-neurofilament 200 kDaantibody (NF200; blue) at the nerve terminal. (B) Staining with IgManti-GalNAc-GD1a mAb (green) was not overlapped with theSchwann cell (glial cell) marker, anti-S100 protein antibody-1(S100; blue) (C) Staining with IgM anti-GalNAc-GD1a mAb(green) was not overlapped with anti-syntaxin antibody (Syn; blue).The bars represent 5 μm.