Figure 3: Immunostaining with IgM anti-GalNAc-GD1a mAb incross-sections of rat hemi-diaphragm after collagenase andchloroform/methanol treatment. (A) Following collagenasetreatment, which dissociates the motor nerve, IgM anti-GalNAc-GD1a mAb (green) and anti-neurofilament 200 kDa antibody(NF200; blue) did not stain these sections. However, thepostsynaptic acetylcholine receptor cluster marker, α-bungarotoxin(α-BuTx), did stain these sections (red). (B) Following chloroform/methanol treatment, which dissociates glycolipids and nerve fibers,IgM anti-GalNAc-GD1a mAb did not stain these sections, but α-BuTx (red) and NF200 (blue) did. The bars represent 5 μm.