Figure 3: CSF reactivity against cerebrosides. (A) The CSF reactivity of 28 MS with OCBs patients was tested in an ECL-based assay against PC, PC/ Chol/Cer and PC/Cer liposomes. The reaction against PC/Cer and PC/Chol/ Cer liposomes was significantly higher as compared with PC liposomes (*p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA). The CSF reactivity of 28 MS with OCBs patients against PC/Chol/Cer (B) and PC/Cer (C) liposomes correlated with the reactivity against myelin liposomes (Pearson correlation test). (D) The reactivity of CSF of 28 MS patients with OCBs patients against myelin particles from wild-type, CerS2 knockout mice myelin and non-myelin was assessed using the ECL-based assay system. CSF reactivity towards wild-type myelin particles was significantly higher as compared with CerS2 knockout myelin and non-myelin (*p < 0.05; one-way ANOVA). (E) Significantly higher reactivity towards wild-type as compared with CerS2 knockout myelin liposomes (***p < 0.001; one-way ANOVA).