Figure 5: Unfoldin localization in vivo. Yeast cells were cultured to an optical density (OD) value at 600 nm of 0.05–0.1 (middle panels), 0.5–0.8 (upper panels), or 5–8 (lower panels) at 30°C to immunostain endogenous Unfoldin. Immunofluorescence staining was performed using standard protocols, and the anti-Unfoldin specific antibody was used at a 1:100 dilution. FITC-conjugated goat anti rabbit IgG was used as the secondary antibody at a 1:200 dilution. Each sample was examined with Delta-Vision microscopy. Out-of-focus light was removed by interactive deconvolution.