| Purification Step |
Total protein(mg) |
Total activity(units)* |
Specific activity(Units/mg) |
Purification(fold) |
Yield(%) |
| NaCl Extract |
200 |
2200 |
11 |
1 |
100 |
| DEAE-FastFlow |
20 |
1066 |
53 |
5 |
49 |
| ResourceQ |
3 |
1000 |
333 |
30 |
45 |
| Hydroxy Apatite |
0.15 |
350 |
2333 |
212 |
16 |
| ATPagarose |
0.03 |
222 |
6938 |
631 |
10 |
One unit is defined as the amount of factor that produces a 50% reduction in the control alpha-factor precursor band under the assay conditions |
