(A) The construction of the plasmid (pcDNA3.1-GFP-PrP). The prion protein has the signal sequence for the secretion at the NH2-terminal (amino acid 1-22) and GPI-anchor signal at the COOH-terminal (amino acid 231-254). GFP insertion is indicated.
(B) Comparison of the subcellular distribution pattern of transiently expressed GFP-PrP (left panel) and stably expressed GFP-PrP (right panel).
(C) Localization of GFP-PrP in stably expressed cells with (right panel) or without (left panel) NGF. Scale bars; 15 μm.
