Figure 5: PDGF-AA treatment promotes oligo-lineage differentiation. Immunohistochemistry against PDGFRa (oligo-lineage, A-D), B3-Tub (neuronal lineage, E-H), GFAP (astrocyte lineage, I-L), and O4 (Mature oligo lineage, M) was used to determine how PDGF-AA induces differentiation after 1 week of treatment. All images represent a representative confocal field of view, and quantification (D, H, I) is presented as the total number of positively stained cells over the total number of cells per field, counted by the nuclear marker Hoechst. At one week, nearly all cells are differentiated, as determined by the absence of the neural stem cell marker nestin (I, J, K). *significance, p<.05.