Figure 3: Phase contrast microscopy and indirect fluorescent immuno-histochemistry of BPNM. A and D are phase contrast micrographs of paraformaldehydefixed BPNM smears, demonstrating aggregates of purified myelin, while B and E demonstrate galactocerebroside and sulfatide immunoreactivity of these smears respectively (original magnification 100X). Partial phase contrast-fluorescent image composites confirm the localization of galactocerebroside (C) and sulfatide (E) to the myelin membrane (white arrows, original magnification 400X).