Figure 5: The thrombin-aptamer complexes, fixed in non-denaturating 8% PAGE. The gel fragments are presented for ease of comparison.
a) RE31 – 100 nM;1 – 0; 2 – 40 nM; 3 -60 nM; 4 – 80 nM; 5 – 100 nM;
6 – 120 nM; 7 – 140 nM; 8 – 160 nM thrombin;
b) RE29-200 nM; 1 – 0; 2 – 40 nM; 3 -60 nM; 4 – 80 nM; 5 – 100 nM;
6 – 120 nM; 7 – 140 nM; 8 – 160 nM thrombin;
c) RE27-200 nM; 1 – 0; 2 – 40 nM; 3 -60 nM; 4 – 80 nM; 5 – 100 nM;
6 – 120 nM; 7 – 140 nM; 8 – 160 nM thrombin;
d) RE25- 200 nM; 1 – 0; 2 – 120 nM; 3 – 180 nM; 4 – 240 nM; 5 – 300
nM; 6 – 360 nM; 7 – 400 nM; 8 – 400 nM thrombin;
e) R23 – 200 nM; 1 – 0; 2 – 120 nM; 3 – 180 nM; 4 – 240 nM; 5 – 300
nM; 6 – 360 nM; 7 – 400 nM; 8 – 400 nM thrombin;
f) RE21 – 200 nM; 1 – 0; 2 – 120 nM; 3 – 180 nM; 4 – 240 nM; 5 – 300
nM; 6 – 360 nM; 7 – 400 nM; 8 – 400 nM thrombin;
g) RE19 – 250 nM; 1 – 0; 2 – 120 nM; 3 – 180 nM; 4 – 240 nM; 5 – 300
nM; 6 – 360 nM; 7 – 400 nM; 8 – 400 nM thrombin;
h) RE15 – 300 nM; 1 – 0; 2 – 240 nM; 3 – 360 nM; 4 – 480 nM; 5 – 600
nM; 6 – 720 nM; 7 – 840 nM; 8 – 960 nM thrombin;