![]() |
Figure 5: The thrombin-aptamer complexes, fixed in non-denaturating 8%
PAGE.
The gel fragments are presented for ease of comparison. a) RE31 – 100 nM;1 – 0; 2 – 40 nM; 3 -60 nM; 4 – 80 nM; 5 – 100 nM; 6 – 120 nM; 7 – 140 nM; 8 – 160 nM thrombin; b) RE29-200 nM; 1 – 0; 2 – 40 nM; 3 -60 nM; 4 – 80 nM; 5 – 100 nM; 6 – 120 nM; 7 – 140 nM; 8 – 160 nM thrombin; c) RE27-200 nM; 1 – 0; 2 – 40 nM; 3 -60 nM; 4 – 80 nM; 5 – 100 nM; 6 – 120 nM; 7 – 140 nM; 8 – 160 nM thrombin; d) RE25- 200 nM; 1 – 0; 2 – 120 nM; 3 – 180 nM; 4 – 240 nM; 5 – 300 nM; 6 – 360 nM; 7 – 400 nM; 8 – 400 nM thrombin; e) R23 – 200 nM; 1 – 0; 2 – 120 nM; 3 – 180 nM; 4 – 240 nM; 5 – 300 nM; 6 – 360 nM; 7 – 400 nM; 8 – 400 nM thrombin; f) RE21 – 200 nM; 1 – 0; 2 – 120 nM; 3 – 180 nM; 4 – 240 nM; 5 – 300 nM; 6 – 360 nM; 7 – 400 nM; 8 – 400 nM thrombin; g) RE19 – 250 nM; 1 – 0; 2 – 120 nM; 3 – 180 nM; 4 – 240 nM; 5 – 300 nM; 6 – 360 nM; 7 – 400 nM; 8 – 400 nM thrombin; h) RE15 – 300 nM; 1 – 0; 2 – 240 nM; 3 – 360 nM; 4 – 480 nM; 5 – 600 nM; 6 – 720 nM; 7 – 840 nM; 8 – 960 nM thrombin; |