| 15TBA |
RE19 |
RE21 |
RE23 |
RE25 |
RE27 |
RE29 |
RE31 |
| 39 ± 0,5 |
33 ± 0,5 |
25 ± 0,5 |
20,5 ± 0,5 |
17 ± 0,5 |
27 ± 0,5 |
32 ± 0,5 |
37,5 ± 0,5 |
The CD values were recorded at 294 nm, melting temperatures are in ˚C . The
attachment of additional oligonucleotide sequences to the G-quadruplex destabilizes
its structure. On the other hand, the greater the number of complementary pairs
formed by these sequences, the higher the stability of the G-quadruplex domain
in a given aptamer. This domain is the least stable in aptamer RE25, where five
pairs of nucleotides are attached to it. Three of them can form a duplex, but its
stability is low. |
