Figure 3: Immunofluorescent staining of the MAS receptor (MAS; green), ACE2 (red), and a nuclear marker (DAPI; blue) in kidney specimens (A, B, C).MAS staining was observed in both the tubules and glomeruli (A, B, C). Merged images of MAS and ACE2 (yellow) staining showing the co-localization of ACE and ACE2 in the proximal tubules of a healthy control and an MCNS patient (A, C). The merged imaged of the proximal tubules of the diabetic nephropathy patient do not show yellow signals (B) reflecting weak ACE2 and MAS staining.