a. Lymphocytes were initially gated according to forward- and side-scatter height (and here identified by the circle in the dot plot). b. CD3+ T cell subset was identified in the lymphocyte area (and here identified by the circle in the dot plot). c. Subsequently γδ expression was examined in this population; the percentage of γδ T cells was obtained in the upper right quadrant in the dot plot.
Figure 1: Gating strategy for γδ T cells.