Sequential Fractions

Lysis Buffer
(ml)

Protein Con.
(mg/ml)

Total Protein
(mg)

Percentage a)

Fraction A

7.8

4.05

31.59*

38%*

Fraction B

5.2

4.13

21.48

26%

Fraction C

5.2

4.93*

25.64*

31%*

a)This percentage was calculated with no consideration of fraction D since there were only trace amounts of proteins in that final fraction. * P<0.05, compared to fraction B, t-test.
Table 1: Protein yields in different extraction fractions.