Intensity order No.

Protein name

Swiss_Prot entry

GRAVY

 

a)

SP

 

b)

TMH
c)

HR

d)

Localization (Component)

Function

a1

Tubulin, alpha

TBA1A_RAT

-0.229

0

0

0

Cytoplsmic

Skeleton protein

a2

Peptidylprolyl isomerase A

PPIA_RAT

-0.326

0

0

0

Cytoplasmic

Protein folding

a3

Calmodulin

CALM_RAT

-0.654

0

0

0

Cytoplasmic

Signal transduction

a4

Actin, beta

ACTB_RAT

-0.2

0

0

0

Cytoplasmic

Skeleton protein

a5

Heat shock protein 8

HSP7C_RAT

-0.452

0

0

0

Cytoplasmic/nuclear

Chaperone

a6

Calretinin

CALB2_RAT

-0.664

0

0

0

Cytoplasmic

Signal transduction

a7

Serum  albumin

ALBU_RAT

-0.389

1

0

1

Secreted

Transporter

a8

Toad-64

DPYL2_RAT

-0.267

0

0

0

Cytoplasmic or membrane-bound

Axon elaboration

a9

Enolase 1

ENOA_RAT

-0.198

0

0

0

Cytoplasmic or membrane-bound

Glycolytic enzyme

a10

Creatine kinase-b

KCRB_RAT

-0.466

0

0

0

Cytoplasmic

Energy transporation

a11

Lactate dehydrogenase

LDHB_RAT

0.033

0

0

1

Cytoplasmic

Glycolytic enzyme

a12

Pyruvate kinase

KPYM_RAT

-0.096

0

0

0

Cytoplasmic

Glycolytic enzyme

a13

Malate dehydrogenase

Q6PCV2_RAT

-0.059

0

0

1

Cytoplasmic

Energy metabolism

a14

Aldolase C

ALDOC_RAT

-0.21

0

0

0

Cytoplasmic

Glycolytic enzyme

a15

Aldolase C

ALDOC_RAT

-0.21

0

0

0

Cytoplasmic

Glycolytic enzyme

a16

Glutamine synthetase 1

GLNA_RAT

-0.601

0

0

0

Cytoplasmic

Amino acid metabolism

a17

Toad-64

DPYL2_RAT

-0.267

0

0

0

Cytoplasmic or membrane-bound

Axon elaboration

a18

Serum  albumin

ALBU_RAT

-0.389

1

0

1

Secreted

Transporter

a19

Heat shock protein 8

HSP7C_RAT

-0.452

0

0

0

Cytoplasmic or nuclear

Chaperone

a20

Cofilin 1

COF1_RAT

-0.388

0

0

0

Cytoplasmic or nuclear

Structural protein

a21

Phosphatidylethanolamine binding protein

PEBP1_RAT

-0.582

0

0

0

Cytoplasmic or membrane-bound

Signal transduction

a22

Peroxiredoxin 6

PRDX6_RAT

-0.225

0

0

0

Cytoplasmic

Redox regulation

a23

Neuronal rhoa gef protein

Q6RFZ7_RAT

-0.537

0

0

0

Cytoplasmic

Signal transduction

a24

Phosphoglycerate mutase 1

PGAM1_RAT

-0.506

0

0

0

Cytoplasmic

Glycolytic enzyme

a25

Triosephosphate isomerase 1

TPIS_RAT

-0.121

0

0

0

Cytoplasmic

Glycolytic enzyme

a26

Triosephosphate isomerase 1

TPIS_RAT

-0.121

0

0

0

Cytoplasmic

Glycolytic enzyme

a27

Peroxiredoxin 2

PRDX2_RAT

-0.175

0

0

1

Cytoplasmic

Redox regulation

a28

Glyceraldehyde 3-phosphate-dehydrogenase

G3P_RAT

-0.084

0

0

0

Cytoplasmic

Glycolytic enzyme

a29

Superoxide dismutase [cu, zn]

SODC_RAT

-0.419

0

0

0

Cytoplasmic

Redox regulation

a30

Nucleoside diphosphate kinase b

NDKB_RAT

-0.27

0

0

0

Cytoplasmic or membrane-bound

Neuclear acid metabolism

a) GRAVY, Grand average of hydropathicity; b) SP, signal peptide; c) TMH, transmembrane helix; d) HR, hydrophobic region.

Table 4: The top 30 most abundant protein spots in soluble fraction A.