Figure 2: Total protein extracts from the MCF-7 cell line were separated by 2-DE and visualized by silver staining (Hamrita et al., 2008). The location of proteins reacting with patient sera is indicated with arrows on the 2-DE gel. 1: enoyl-CoA hydratase; 2: breast cancer patient sera is indicated with arrows on the 2-DE gel. 1: enoyl-CoA hydratase; 5: cytokeratin 8; 6: hnRNPA2B1; 7: HSP60; 8: peroxiredoxin-2; 9,10: hnRNPH; 11: cytokeratin 18; 12,13: cytokeratin 8; 14: β-tubulin; 15: prohibitin; 16: hnRNPK; 17: protein disulfide isomerase; 18: hnRNPK; 19: nucleoside diphosphate kinase A; 20: hnRNPH3; 21: transitional endoplasmic reticulum ATPase; 22: F-actin; 23: ornithine aminotransferase; 24: Mn-SOD; 25: haptoglobin-related protein; 26: Bcrystallin.