Figure 3: Identification of reactive protein spot as HSP60 by MALDI-TOF mass spectrometry and by western blot analysis (Hamrita et al., 2008). Upper panel: MALDI-MS spectrum obtained after trypsin digestion and peptide sequences from HSP60 matching with peaks obtained from MALDI-MS spectra (bottom).Lower panel: Close-up of a Coomassie bluestained 2-DE gel in the location of HSP60 protein is shown (1). MCF-7 cell lysate proteins were separated by 2-DE, transferred to PVDF membranes and then immunoblotted with either a sera from a breast cancer patient (2) and from a healthy individual (3) or with an anti-HSP60 antibody (4).