Residue
pKa
pH7
pH5
ASP127
5.7
-
+
ASP380
7.14
+
+
GLU233
9.28
+
+
GLU340
5.24
-
+
GLU481
5.06
-
+
HIS60
6.25
-
+
HIS145
6.5
-
+
HIS162
7.34
+
+
HIS223
6.43
-
+
HIS274
6.43
-
+
HIS290
6.43
-
+
HIS328
6.43
-
+
HIS311
7.49
+
+
HIS495
6.5
-
+
NOV
8.69
+
+
Table 1:
Predicted pKa values and protonation states of ionizble residues and NOV (+: protonated). Aspartic acid, glutamic acid and histidine residues which are protonated at pH 5 are shown.
