Figure 1: Flowchart for the large-scale identification and characterization of phosphorylated proteins and sites in the gastric cancer cells. The cells were serum-deprived for 12 h, followed by treatment with 1 mM Na3VO4 and 5 mM NaF at 37oC for 30 min. 5mg of Cellular lysate was separated by 10% SDSPAGE; then stained with Coomassie blue R-250.