Figure 1: Immunodepletion of patient plasma. The depletion of highly abundant proteins was achieved by Multi Affi nity Reverse Spin (MARS) Column in a bound fraction and the low abundant proteins were retained in the unbound or fl ow through fraction. After the incubation period, unbound proteins were centrifuged and collected directly into a spin concentrator 5 kDa molecular weight fi lter (Agilent). The bound fraction was eluted with elution buffer into another spin concentrator fi lter. The total amount of protein in depleted (fl ow-through fraction) and abundant plasma proteins (bound fraction) was determined using a Bradford protein assay and run on a SDS-PAGE. Lane 1: Protein molecular weight marker; lane 2: Crude human plasma; lane 3: bound fraction and lane 4: fl ow through fraction after immunodepletion. Results are a representative experiment from three independently performed experiments with similar results.