Figure 1: Two-dimensional gel electrophoresis images of MCF-7 cell and mouse liver tissue proteomes isolated by different protein isolation methodologies. Aqueous phase proteins isolated by using chloroform-assisted protein isolation (ChlAPI) method from MCF-7 cells (A) and mouse liver tissue (D). MCF-7 cell total soluble proteome isolated by detergent lysis followed by sonication (B), and by French Pressure Cell Press(C).