Sample

Total proteins

identified

(<1% FDR)

No. of   proteins

annotated by

GO

 

Membrane

proteins (%)

Control, aqueous fraction

752

 

376

 

11.7

 

Control, organic fraction

 

593

 

356

 

39.6

 

E2-treated, aqueous fraction

 

649

 

340

 

15.0

 

E2-treated, organic fraction

 

712

 

403

 

40.2

 

Control total (aqueous + organic)

 

1134

 

589

 

29.5

 

E2-treated total (aqueous +

organic)

 

1145

 

594

 

30.5

 

Common (control and E2 treated)

879

480

29.0

GO = Gene Ontology; FDR = False Discovery Rate

Table 1: MCF7 cell proteinsidentified after chloroform-assisted protein isolation (ChlAPI) and mass spectrometry.