Figure 5: Structural superpositions between protein 10961_61 and diguanylate cyclases. (A.) The structures of the model constructed for protein 10961_61 (backbone thickened) and diguanylate cyclase from Pseudomonas aeruginosa pao1 (backbone thinned, PDB: 3bre chain B) are superimposed and colored by the distance deviation of the corresponding C-alpha atoms (2nd bar in (B)). The 108-EPGLF-112 sequence from protein 10961_61, which corresponds to the GGEEF sequence motif of the active site from 3bre is colored in blue. Positions that correspond to selected residues from the allosteric inhibitory site (I-site) in 3bre (De et al. 2008) are indicated by violet spheres. (B.) Structure-based sequence alignment (top; fragment: 29-VRDD...ERIL-131), and bar representation of deviations in structural alignment (bottom) of protein 10961_61 with diguanylate cyclases from Caulobacter vibrioides (PDB: 1w25), Pseudomonas aeruginosa pao1 (PDB: 3bre), and Geobacter sulfurreducens (PDB: 3ezu). Distance deviations are calculated using model of 10961_61 as a frame of reference. Distance deviations between superimposed corresponding residues are indicated using the same color scheme as in Figure 4B.