Figure 3: SDS-PAGE of recombinant proteins before and after enzymatic proteolysis. [A] 1D SDS-PAGE of recombinant Fibrillarin protein. Lane 1: full length protein. Lane 2: band of trypsin-truncated Fibrillarin. [B] 1D SDS-PAGE of recombinant RA33 protein. Lane 1: full length protein. Lane 2: band of trypsin proteolysis products of RA33 are circled. [C]: 1D SDS-PAGE of recombinant RA33 protein. Lane 1: full length protein. Lane 2: bands of thermolysin proteolysis products of RA33 are circled. Molecular mass marker positions are indicated on the left (numbers refer to apparent molecular masses in kDa).