Spot Protein Sequence coverage (%) ncbi ID Theoretical value Experimental value Changes pH Mr (kDa) pH Mr (kDa) RP : PP S1 Hypothetical protein 36 NP_065189.1 6.2 22.1 7 36 (-) : (+) S2 Glyceraldehyde-3-phosphate dehydrogenase 34 CAA25833.1 8.4 36.21 8 45 (+) : (-) S3 Single recognition particule (54kDa) 17 NP_003127.1 9 55.97 7 150 (+) : (-) S4 ACTB protein 25 AAH12854.1 5.6 40.54 6 60 (+) : (-) S5 Similar to human albumin 9 AAA64922.1 5.7 53.43 7.5 60 (+) : (-) S6 STAG1 protein 10 AAG64699.1 5.3 141.04 3.5 90 (-) : (+) S7 Sarcolectin 22 CAB41416.1 5.6 51.45 4 80 (-) : (+) S8 Arginiosuccinate synthetase 1 30 NP_446464.1 8.3 46.8 9 60 (+) : (-) S9 Proteasome activator hPA28 subunit beta 52 BAB08205.1 5.4 27.5 5.5 40 (-) : (+) S10 Hypothetical protein 10 CAH10535.1 5.8 55.73 8 45 (+)   (-) S11 Transkeratolase 28 NP_001055.1 7.8 68.54 8.5 100 (+) : (-)

Table 1: Differentially expressed proteins identified by proteomics from recurrent pterygium tissue obtained. S1 to S11 represent ID number of spots and sequence coverage, and the theoretical value of pI and Mr were obtained from the Pro Found search. The calculations of the experimental pI and Mr were based on the migration of the protein on a 2D gel. (RP : recurrent pterygium, PP: primary pterygium).