Figure 2: SDS-PAGE analysis of secreted proteins prepared with modified sevage method. Protein standards were marked beside the gel (Molecular masses in kDa). Each lane load 8 µg proteins measuring by Bradford assay. The gel was sliver stained. Lane1, lane3, lane5, lane7, lane9, lane11 and lane13 represented the secreted proteins from EP155 and the culture time was one to seven days with 24-hour interval. Lane2, lane4, lane6, lane8, lane10, lane12 and lane14 represented the secreted proteins from EP713 and the culture time was one to seven days with 24-hour interval.