Sequence

Mouse Liver

Mouse Brown Adipose

Site  -5  -4  -3  -2  -1 0 +1 +2 +3 +4 +5

Fed [3]

4 hr fast [2]

20 hr fast [1]

4°C [1]

RT [1]

None* [1]

Fed [2]

4 hr fast [0]

20 hr fast [3]

4°C [2]

RT [1]

M1                                M    E   V   H   E     L  

xxx

xx

x

x

x

x

xx

 

x

x

x

K52    R     P   K   A    L   K   P   P   C     D   L

 

 

 

 

 

   

x

 

 

 

 

K223  L     T   P   C    L   K   I    I   V      I    M

x

 

 

 

 

 

x

 

 

 

x

K241  V     E   R   G    K  K   C   G   V     E   I

 

 

 

 

 

 

 

 

x

 

 

K250  E     I   I    S     L  K   A   L    E     D   L

 

 

 

 

 

 

 

 

x

 

 

K387  A     N  T    S    L   K   R   W L      L   D

 

 

 

 

 

 

 

 

 

 

x

K428  S     S   L   G    G  K   V   R   L      M   I

 

 

 

 

 

 

x

 

xx

 

x

K562  K     K   H  I      F  K   L   A   Q      G  E

xx

x

x

x

x

 

x

 

x

 

x

K641  L     D   D  L     L  K   G   K   N      A   G

 

 

 

 

 

 

x

 

 

 

 

K649  K     N  A   G    L  K    P   F   E      Q   V 

x

 

 

 

 

 

 

 

 

 

 
x, Identified acetylated lysines. Each “x” indicates that the modification was identified and multiple “x’s” indicate that the modification was identified by the corresponding number of replicates. For example, “xx” indicates that the modification was identified in 2 replicates.
[ ] Number of replicates.
* Endogenous ACSL1 isolated from differentiated brown adipocytes
Abbreviations: RT, room temperature
Note: ACSL1 was immunopurified, using gentle lysis buffer, from liver crude mitochondria, except for one of the 4 hr fasting liver samples (which was a lysate), and from brown adipose tissue lysates.
Table 2: Acetylation sites found in endogenous ACSL1 from mouse liver or brown adipose tissue.