Figure 4: Association of cytoplasmic peptidyl Cys with functional networks according to reduction and oxidation of Cys residues. Percent reduction values for the 20% most reduced Cys (Panel A) and the 20% most oxidized Cys (Panel B) were analyzed by Ingenuity Pathway Analysis (IPA). In this analysis, symbols with colors represent proteins associated with peptides identified with percent oxidation data in Supplement 1. Open symbols represent components of the functional network for which data were not available. Asterisks indicate that multiple identifiers in the dataset file map to a single gene/chemical in the global molecular network. In A, the top network associations of the most highly reduced Cys were with cancer, gastrointestinal disease, cellular assembly and organization and protein synthesis (Score 58). A large number of these proteins are associated with the cytoskeleton. In B, the most oxidized peptides had network associations with cancer, cell signaling and post-translational modification (Score 51). Abbreviations: ACTB, beta-actin; ACTN4, alpha-actinin-4; AIMP2, aminoacyl tRNA synthase complex-interacting multifunctional protein 2; ALDOA, fructose-bisphosphate aldolase A; AMPK, 5’-AMP-activated protein kinase; ANXA1, annexin A1; ANXA4, annexin A4; ATP1A1, sodium/potassium-transporting ATPase subunit alpha-1; CAND1, cullin-associated NEDD8-dissociated protein 1; CAP1, adenylyl cyclaseassociated protein 1; CCT4, T-complex protein 1 subunit delta; CCT5, T-complex protein 1 subunit epsilon; CCT7, T-complex protein 1 subunit eta (TCP-1-eta); CLTC, clathrin heavy chain 1; CTPS, CTP synthase 1; DIAPH1, protein diaphanous homolog 1; EEF1B2, eukaryotic elongation factor 1-beta; EIF2S2, eukaryotic translation initiation factor 2 subunit 2; EIF2S3, eukaryotic translation initiation factor 2 subunit 3; EPCAM, epithelial cell adhesion molecule; EPRS, bifunctional aminoacyl-tRNA synthetase; FASN, fatty acid synthase; FKBP4, peptidyl-prolyl cis-trans isomerase; FLNA, filamin A; FLNB, filamin B; G6PD, glucose 6-phosphate 1-dehydrogenase; HSPH1, heat shock protein 105 kDa; LDHA, L-lactate dehydrogenase A chain; LDHB, L-lactate dehydrogenase B chain; MIF, macrophage migration inhibitory factor; MIR1, E3 ubiquitin-protein ligase; MYO1C, myosin-Ic (myosin I beta); PABPC4, polyadenylate-binding protein 4; PEBP1, phosphatidylethanolamine-binding protein 1; PGK1, phosphoglycerate kinase 1; PLA2, phospholipase A2; PLEC, plectin; PP1A, serine/threonine-protein phosphatase PP1-alpha catalytic subunit; RPS3, 40S ribosomal protein S3; STK39, STE20/SPS1-related proline-alanine-rich protein kinase; TCP1, T-complex protein 1 subunit alpha; TUBA1A, tubulin alpha-1A chain; TUBA4A, tubulin alpha-4A chain; TUBB, tubulin beta; UBA1, ubiquitin-like modifier-activating enzyme 1; YWHAH, 14-3-3 protein eta; YWHAZ, 4-3-3 protein zeta/delta.