Figure 3: Subnuclear localization of GFP-tagged Ddx5 and Ddx5MUT proteins.
A.
Schematic representation of GFP-tagged Dnmt3a1, Dnmt3a2, Ddx5-Nter, Ddx5-Cter, RFP-tagged Ddx5 and Ddx5MUT and Gal4(DBD)-Dnmt3a2 proteins. Numbers indicate amino acid positions.
B. Fluorescence microscopy of EGFP-Ddx5 (Top) and Ddx5MUT (Bottom) proteins. White arrows indicate EGFP-Ddx5 nuclear speckles.
C.
Confocal microscopy of EGFP-Ddx5Nter and EGFP-Ddx5Cter proteins.