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Figure 3: Subnuclear localization of GFP-tagged Ddx5 and Ddx5MUT proteins. A.Schematic representation of GFP-tagged Dnmt3a1, Dnmt3a2, Ddx5-Nter, Ddx5-Cter, RFP-tagged Ddx5 and Ddx5MUT and Gal4(DBD)-Dnmt3a2 proteins. Numbers indicate amino acid positions. B. Fluorescence microscopy of EGFP-Ddx5 (Top) and Ddx5MUT (Bottom) proteins. White arrows indicate EGFP-Ddx5 nuclear speckles. C. Confocal microscopy of EGFP-Ddx5Nter and EGFP-Ddx5Cter proteins. |