Figure 3: Decreased expression of FAS and CPS-1, and increased expression of KIAA0664 in mouse liver due to high fat feeding were confirmed by corroborative methods. The mouse liver tissues were homogenized and the proteins were resolved by SDS-PAGE. A. For FAS, the gel was stained with Coomassie Blue and the bands were validated as FAS by mass spectrometry. B. For CPS-1, the gel was stained with Coomassie Blue and the bands were validated as CPS-1 by mass spectrometry. C. For KIAA0664, the gel was subjected to Western blotting and probed with anti-KIAA0664 and anti-beta actin antibodies.