Discriminant peptide* peak m/z value Monoisotopic Peak Number Preliminary protein identification # Method  1, 2 or 3 3277.7 42a Fibrinogen ad    2 3 3261.6 41a Fibrinogen aa 1 2 3 3222.5 40a     2 3 3190.6 38a Fibrinogen aa      3 3156.8 37a Inter-a-trypsin inhibitor fragments b    2 3 2931.5 35a Fibrinogen aa  1 2 3 2768.4 33a Fibrinogen aa  1   3 2753.6 32a Albumin precursor (25-28) d 1 2 3 2553.3 29a Fibrinogen  a a 1   3 1740.1 25a C4a a    2   1616.8 24a Fibrinopeptide Ab 1 2 3 1561.9 23a C3f a 1 2 3 1533.2 21a   1   3 1518.8 20a Fibrinopeptide A-H20 d 1 2 3 1487.7 18a     2   1465.8 17a Fibrinopeptide A a  1     1418.7 14a   1     1347.6 11a C3f a 1 2   1260.6 9a   1 2 3 1206.6 6a Fibrinopeptide A a,c   1 2   1077.5 4a Fibrinopeptide A a   2   1039.6 3a       3 1020.5 2a Fibrinopeptide A a,c 1     *determined from weighting coefficients generated from LRA, for each method top 15 weighting coefficients were selected #as determined from the publications of (a) Villanueva et al. [26] (b) Hortin et al. [24], (c) Gianazza et al. [23] and (d) Tiss et al. [25].

Table 2: Discriminant peptides* of found in different data handling strategies. The table shows the top 15 discriminant peptides* for each of the three methods, its related monoisotopic peak number, preliminary protein identification# and the overlap between the 3 methods.