Spot No. Name of the Protein/Polypeptide Accession Id. Mr pI Mascot Score Sequence Coverage No. of MS/MS matches
1. Serum Albumin-Human 1BKE 65,993 5.69 191(64) 64% 9
2. Alpha-1-B-Glycoprotein-Human Q68CK0_HUMAN 54,220 5.56 160(64) 48% 7
3. Alpha-1-antitrypsin-Human AAB59495 46,677 5.43 110(64) 36% 8
4. Vitronectin precursor-Human SGHU1V 54,328 5.55 112(64) 23% 5
5. Kininogen, HMW precursor KGHUH1 71,900 6.34 115(64) 40% 6
6. Prothrombin-Human Q4QZ40_HUMAN 69,920 5.70 202(64) 48% 8
7. Plasma protease C1 inhibitor (fragment)-Human Q59EI5_HUMAN 56,695 5.98 116(64) 27% 7
8. Complement C1 inhibitor precursor-Human ITHUC1 55,119 6.09 105(64) 29% 5
9. Vitamin D binding protein-Human Q53F31_HUMAN 52,916 5.34 221(64) 51% 8
10. Alpha-2-HS-glycoprotein precursor-Human WOHU 39,300 5.43 158(64) 40% 5
11. Haptoglobin precursor-Human HPHU1 38,427 6.13 242(64) 29% 5
12. Human apolipoprotein-A-IV AAA51748 43,358 5.22 494(64) 61% 9
13. Complement component C3d-Human 1C3D 32,845 6.34 219(64) 44% 6
*14. Haptoglobin precursor HPHU1 38,427 6.13 75(64) 14% 1
*15. Complement component C3b-Human S27041 25,280 4.49 49(64) 55% 4
16. a1-microglobulin HCHU 38,974 5.95 95(64) 35% 5
17. APOA1 protein (fragment)-Human CAA00975 28,061 5.27 470(64) 81% 11
18. Serum amyloid P-Human YLHUP 25,371 6.10 181(64) 30% 4
19. Preproapolipoprotein A1-Human LPHUA1 30,759 5.56 173(64) 59% 7
20. Transthyretin chain A-Human 2TRYA 13,829 5.35 216(64) 94% 5
21. Human a fetoprotein E973181 66,358 5.67 264(64) 49% 6
22. Transferrin-Human Q53H26_HUMAN 77,030 6.68 339(64) 51% 8
23. Fibrinogen b chain-Human FGHUB 55,892 8.54 592(64) 49% 10
24. Fibrinogen α chain-Human FGHUA 69,714 8.23 508(64) 38% 8
25. Fibrinogen b chain fragment d-Human 1FZAB 35,875 7.66 253(64) 79% 5
26. Voltage gated Ca channel a2d3 subunit-Human Q8IZS8_HUMAN 122,933 5.53 65(64) 29% 0
27. Fibrinogen α chain extended splice form-Human D44234 94,914 5.70 204(64) 35% 4
28. Immunoglobulin k light chain VLJ region-Human BAC01677 27,574 7.53 160(64) 33% 3
*29. Immunoglobulin k chain V-III region (B6)–Human K3HUB6 11,628 9.34 59(64) 16% 1
30. Haptoglobin precursor HPHU2 45,177 6.13 71(64) 53% 5
31. Immunoglobulin k chain  NIG26 precursor-Human JEO242 23,504 5.46 203(64) 48% 3
*32. Immunoglobulin l light chain variable region (fragment)-Human AAD16673 11,505 5.67 32(64) 25% 1
33. Hemoglobin a chain (fragment)-Human Q9BX3_HUMAN 10,703 7.07 87(64) 60% 3
34. Hemoglobin b chain-Human 2HBSB 15,827 7.26 143(64) 82% 6
35. Ig κ light chain VLJ region (fragment)-Human BAC01701 29,183 8.84 127(64) 39% 4
36. Anti RhD monoclonal T125 k light chain precursor-Human Q5EFE6_HUMAN 25,682 8.70 56(64) 41% 2
37. Fibrinogen fragment d, chain C-Human 1FZEC 34,457 5.68 64(64) 56% 2
38. Fibrinogen fragment d, chain B-Human 1FZAB 35,875 7.66 137(64) 53% 5
39. Fibrinogen fragment d, chain F-Human 1FZEF 34,343 5.68 109(64) 54% 4
40. Fibrinogen fragment d, chain C-Human 1FZAC 35,144 5.57 91(64) 66% 3
41. Fibrinogen beta chain precursor FIBB_HUMAN 55,892 8.54 107(53) 48% 5
42. Fibrinogen γ A chain precursor-Human FGHUG 49,465 5.70 313(64) 55% 9
43. ALB protein (Growth inhibiting protein 20)-Human Q86YGO_HUMAN 47,330 5.97 170(64) 39% 8
44. Apolipoprotein-A-IV precursor-Human LPHUA4 45,307 5.23 107(64) 58% 5
45. α1-antitrypsin precursor-Human ITHU 46,707 5.37 248(64) 55% 8
46. Fibrinogen γ B chain precursor-Human FGHUGB 51,479 5.37 343(64) 64% 8
47. Coagulation factor XIII chain b precursor-Human KFHU13 75,442 5.97 94(64) 34% 3
48. Plasminogen-Human Q5TEH4_HUMAN 90,510 7.04 90(64) 40% 6
49. Fibronectin1-Human Q60FE4_HUMAN 252,848 5.66 224(64) 26% 13
50. HUMPIS NID (CDC2-related protein kinase)-Human AAA60092 35,549 9.02 65(64) 52% 1
51. Replication licensing factor MCM2-Human S42228 99,174 5.72 68(64) 21% 0
52. (P02735) Serum amyloid A protein precursor-Human SAA_HUMAN 13,524 6.28 157(64) 52% 2
53. (P01574) Interferon beta precursor (IFN-beta) (Fibroblast interferon)-Human IFNB_HUMAN 22,279 8.93 53(53) 28% 0
54. (P61011) Signal recognition particle 54 kDa protein (SRP54) SRP54_HUMAN 55,668 8.87 53(53) 16% 0
55. Hypothetical protein DKFZp779N0926-Human Q7Z664_HUMAN 45,064 5.76 547(64) 46% 6
**56. Collagen alpha 1(XI) chain precursor - human CGHU1E 181,029 5.11 53(64) 21% 2
57. Nicotinic acetylcholine receptor epsilon chain precursor - human S34775 54,581 5.09 43(64) 16% 1
**58. Glutathione S-transferase A2 GSTA2_HUMAN 25,531 8.54 33(64) 85% 1
59. SET domain, bifurcated 1-Human Q5SZD8_HUMAN 27,685 4.85 45(64) 24% 1
**60. Adenylate Kinase 1-Human Q5T9B7_HUMAN 23,396 8.78 29(64) 29% 2
**61. Interleukin-14 precursor –Human A48203 54,723 9.32 26(64) 27% 1
62. 3' Histone mRNA exonuclease1 THEX1_HUMAN 39,907 6.32 45(64) 43% 1
63. AB009303 NID membrane-type matrix metalloproteinase 3 BAA23742 69,451 8.72 58(64) 34% 1
64. Leucine-rich PPR motif-containing protein-Human Q7Z7A6_HUMAN 157,805 5.81 53(64) 17% 1
**65. BC015875 NID-Human
(Selenoprotein P)
AAH15875 43,157 7.59 51(64) 26% 1
**66. 1-Phosphatidylinositol-4-phosphate 5-kinase-Human A55967 46,163 7.70 24(64) 9% 1
67. HSP63G13 NID (p63 protein)-Human AAG45609 55,652 6.41 60(64) 25% 1
68. (Q9UPY3) Endoribonuclease Dicer-Human DICER_HUMAN 217,490 5.45 47(53) 11% 1
69. (P58340) Myeloid leukemia factor 1 MLF1_HUMAN 30,608 9.46 37(53) 34% 2
70. Matrix metalloprotease MMP-27 Q9H306_HUMAN 58,986 8.83 36(64) 23% 2
*71. Apolipoprotein E precursor (ApoE) APOE_HUMAN 36,132 5.65 284(64) 50% 7
72. T-cell receptor b-chain precursor CAA71260 15,318 6.07 46(64) 43% 1
**73. Homeobox protein CHX10 CHX10_HUMAN 39,386 7.11 33(53) 17% 1
74. Ephrin-A1 precursor EFNA1_HUMAN 23,756 6.49 41(53) 36% 1
75. Intestinal alkaline phosphatase precursor PPBI_HUMAN 56,776 5.53 36(53) 18% 1
76. Ficolin 3 precursor FCN3_HUMAN 32,868 6.20 96(64) 22% 4
77. Complement component C4 fragment Q5ST68_HUMAN 32,378 8.50 146(64) 37% 4
78. Fibrinogen gamma chain precursor FIBG_HUMAN 51,479 5.37 226(64) 30% 6
79. Transthyretin precursor / Prealbumin (multimer) TTHY_HUMAN 15,877 5.52 214(64) 81% 3
80. Adrenocorticotrophic hormone (ACTH) (glycosylation shifts Mr and pI) CAA00890 4,692 8.34 112(64) 78% 1
81. Alpha-2-macroglobulin precursor (Alpha-2-M) A2MG_HUMAN 163,175 6.00 74(53) 18% 2
82. Inter-alpha-trypsin inhibitor heavy chain H4 precursor ITIH4_HUMAN 103,294 6.51 73(53) 26% 3
83. Complement C3 precursor CO3_HUMAN 187,046 6.02 80(53) 25% 3
84. Alpha-1-acid glycoprotein 1 precursor (Orosomucoid 1) A1AG1_HUMAN 23,497 4.93 130(53) 31% 3
85. histidine-rich glycoprotein precursor – human (glycosylation shifts pI) KGHUGH 59,541 7.09 137(64) 22% 4
86. Sodium/hydrogen exchanger 2 (NHE-2) SL9A2_HUMAN 91,461 9.20 62(53) 13% 2
87. C4A2 (C4A3) Q6U2F0_HUMAN 58,393 5.67 77(64) 35% 6
* - protein identifications supported by the published SWISS-2D-PAGE map of human plasma
** - protein identifications supported by other proteomic studies of blood plasma/serum (Ref. 4, 6, 7)
Table 1: Protein Identifications from 20% (NH4)2SO4 precipitate of normal plasma by 2DGE-MALDI ToF/ToF Tandem Mass Spectrometry.