Figure 2: A) Quality of spot reproducibility within slides. Plotting the spot replicates within each slide in a correlation plot with spot replicate one on the x-axis and spot replicate two on the y-axis showed that the spot replicates have a good correlation towards each other (R = 0.98) indicating that the spotting procedure is robust and have good reproducibility and that few spot replicates are needed in order to get reliable results. B) Quality of spot reproducibility between slides. Plotting two slide replicates against each other show good reproducibility between the replicates with a good correlation (R = 0.98) albeit with lower correlation for samples with higher contents of target protein. C) Quality of spot reproducibility between separate prints. 182 samples were reprinted and reanalysed on fluorescent arrays and results from the replicate printings were plotted against each other. This showed comparable results with good correlation (R = 0.9) between printings which indicates that single printings would be sufficient for obtaining data.