Figure 1: (1a) Rehydration buffer-I optimization for leaf proteins of C. dactylon with rehydration buffer-I: Proteins were extracted with phenolic/ ammonium acetate method from C. dactylon leaves and isolated. The sample was solubilized in the rehydration buffer-I and run for IEF according to the conditions as given in the materials and method section. 3 mg of proteins were loaded for IEF separation using immobilized pH gradient strips (3-10). SDSPAGE gel was stained with coomassie brilliant blue. (1b) Optimizing rehydration time: The 2-DE was performed according to the same conditions as for figure 1a with the exception of 14 hrs rehydration time rather 12 hrs.