To confirm some of the findings in tables 1 and 2, human primary T cells were stimulated and DRMs isolated as described in figure 1. Peak raft fractions (combined fractions 2-5) were subsequently subjected to immunoblotting and analyzed for the indicated protein content. Proteins like ILF3 and CIN85 were recruited to the DRMs upon CD3/CD28 triggering (upper panel) whereas integrin 1-alpha/CD11a was displaced from the DRMs upon co-stimulation (lower panel). ZAP-70 and Csk were used as positive controls of recruitment or displacement, respectively. LAT was used as loading control as it constitutively resides in the DRMs of T cells.
