Figure 1: Flow diagram of the process of secondary structural fold class prediction. The flow of the secondary structural protein fold class prediction is described. An 8000 (203) tripeptide library of the probable secondary structure content was built from non-redundant PDB entries. Training sets of all-α, all-β and others (α+β) class members were collected referring SCOP classifications. Relevant PDB entries were used to generate information of the reference classes. LDA was conducted using the covariance matrices of reference classes and the secondary structure content of a query protein. Accuracy analysis as error rate calculation was conducted after the prediction of the secondary structural fold class by LDA.