Figure 4: Validation of HAP2 in various human serum samples by western blotting against anti-human haptoglobin. (A) HAP2 regions of SYPRO® Ruby stained 1-D SDS PAGE gels from individual human serum patients. (B) Western blotting analysis of HAP2 in many human serum samples from healthy and NSCLC patients. Labels: N, normal serum samples (N4-N7, N9, N10-13); L, NSCLC serum samples (Adenocarcinoma (AC) – L1, L7, L10, L15, L19, L22, L23, L29; Squamous cell carcinoma (SQ) – L2, L5, L6, L8, L17; Unknown type of lung cancer (UN) – L4, L12, L18, L25, L26) and M, molecular weight markers are shown to the left. (C) Comparative protein band intensities of HAP2 expression level in normal and NSCLC serum samples.