Spot No. Acc. No.a Observed MW/pI Theoretical MW/pIb Mowse score % Seq. covc Matching peptide sequencesc) N-1 P00738 18000/5.95 41717/6.23 133 8 TEGDGVYTLNDK LRTEGDGVYTLNNEK TEGDGVYTLNNEKQWINK N-2 P00738 18000/5.60 41717/6.23 117 7 TEGDGVYTLNDKK LRTEGDGVYTLNNEK L-1 P00738 18000/5.90 41717/6.23 259 12 YQCKNYYK LRTEGDGVYTLNNEK TEGDGVYTLNNEKQWINK AVGDKLPECEAVCGKPK L-2 P00738 18000/5.65 41717/6.23 645 24 YQCKNYYK NYYKLR TEGDGVYTLNDK TEGDGVYTLNDKK AVGDKLPECEADDGCPKPPEIAHGYVEHSVR LRTEGDGVYTLNNEK TEGDGVYTLNNEKQWINK AVGDKLPECEAVCGKPK LPECEAVCGKPK aAccession number according to Swiss-Prot database. bpI and MW values are calculated by the MASCOT software. cMatching peptide sequences and percentage of amino acid sequence coverage are given by MASCOT for the identified protein.

Table 1: Protein identification of HAP2 by LC-MS/MS analysis.