Figure 5: Hierarchical clustering of the 472 differential protein spots used in the classifier to determine clustering of ‘unknown’ samples. The experimental groups are displayed in rows and the protein spots by column. Differential expression is shown by color, with the red boxes being up-regulated and the green being down-regulated relative to the pooled standard. The unknown samples (Unknown 1, Unknown 2, and Unknown 3) were accurately grouped with their corresponding experimental groups (KIM5 D27 4 mM calcium chloride, KIM5 D27 0 mM calcium chloride, NYC 0 mM calcium chloride), respectively.